Specific binding sites for progesterone and 17β-estradiol in cells of Triticum aestivum L
نویسندگان
چکیده
The presence and location of specific binding sites for progesterone and 17β-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [3H]progesterone and [3H]17β-estradiol occurs in wheat cells. The binding sites are located in membranes and in the cytosol. Specific binding of [3H]17β-estradiol is higher in the membranes than in the cytosol. Specific binding of both ligands in the cytosolic fraction is higher in vernalized plants than in non-vernalized ones. The possibility of the occurrence of steroid binding proteins specific for progesterone and 17β-estradiol, putative steroid receptors for these steroids in Triticum aestivum L., is discussed.
منابع مشابه
Specific binding sites for progesterone and 17beta-estradiol in cells of Triticum aestivum L.
The presence and location of specific binding sites for progesterone and 17beta-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [(3)H]progesterone and [(3)H]17beta-estradiol occurs in wheat cells. The binding sites are located in me...
متن کاملSerum Liver proteins and 17β-estradiol in Postmenopausal Women with Breast Cancer
Background: Breast cancer is a hormone-dependent malignancy that is associated with estrogen and progesterone interactions. The liver is the most important organ to be affected by the metastasis of breast cancer, which causes functional impairment. We compared levels of obesity, 17β-estradiol, and secreted proteins in postmenopausal women with breast cancer but without hepatic symptoms to those...
متن کاملنقش محافظتی استروژن و پروژسترون بر بافت معدی به دنبال ایسکمی- رپرفیوژن معدی در موشهای صحرایی نر
Background and Objective: Ischemia-reperfusion (I/R) injury repeatedly occurs in situations such as surgery, hemorrhage, burns, trauma, and infection. It has also been found that females were more resistant than males, indicating that female sex hormones have a protective function. The aim of this study was to determine the role of estrogen, progesterone, and their combination in protecting the...
متن کاملThe effect of 17β-estradiol on gene expression of calcitonin gene-related peptide and some pro-inflammatory mediators in peripheral blood mononuclear cells from patients with pure menstrual migraine
Objective(s):The neuropeptide calcitonin gene-related peptide (CGRP) has long been postulated to play an integral role in the pathophysiology of migraine. Earlier studies showed that CGRP can stimulate the synthesis and release of nitric oxide (NO) and cytokines from trigeminal ganglion glial cells. The purpose of this study was to determine the effect of 17β-estradiol in regulation of CGRP exp...
متن کاملOccurrence and fate of emerging pollutants of 17beta-estradiol and testosterone in hospital wastewater and effluent: The effect of activated sludge and chlorination processes
Introduction: In 2015, the European Union placed estrogen hormones on the list of compounds with a possible real risk to living organisms and emphasized the need for environmental research. The aim of this study was to determine the occurrence and effect of activated sludge process and chlorination on the fate of 17-beta estradiol and testosterone in hospital wastewater. Materials and Methods: ...
متن کامل